| Chapter title |
Nucleic Acid Aptamers
|
|---|---|
| Chapter number | 4 |
| Book title |
Nucleic Acid Aptamers
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3197-2_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3196-5, 978-1-4939-3197-2
|
| Authors |
Kimoto, Michiko, Matsunaga, Ken-Ichiro, Hirao, Ichiro, Michiko Kimoto, Ken-ichiro Matsunaga, Ichiro Hirao, Matsunaga, Ken-ichiro |
| Abstract |
Genetic alphabet expansion of DNA using unnatural base pair systems is expected to provide a wide variety of novel tools and methods. Recent rapid progress in this area has enabled the creation of several types of unnatural base pairs that function as a third base pair in polymerase reactions. Presently, a major topic is whether the genetic alphabet expansion system actually increases nucleic acid functionalities. We recently applied our unnatural base pair system to in vitro selection (SELEX), using a DNA library containing four natural bases and an unnatural base, and succeeded in the generation of high-affinity DNA aptamers that specifically bind to target proteins. Only a few hydrophobic unnatural bases greatly augmented the affinity of the aptamers. Here, we describe a new approach (genetic alphabet Expansion SELEX, ExSELEX), using our hydrophobic unnatural base pair system for high affinity DNA aptamer generation. |
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Mendeley readers
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| Student > Bachelor | 4 | 15% |
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