Chapter title |
Informing Stem Cell-Based Tendon Tissue Engineering Approaches with Embryonic Tendon Development
|
---|---|
Chapter number | 6 |
Book title |
Metabolic Influences on Risk for Tendon Disorders
|
Published in |
Advances in experimental medicine and biology, August 2016
|
DOI | 10.1007/978-3-319-33943-6_6 |
Pubmed ID | |
Book ISBNs |
978-3-31-933941-2, 978-3-31-933943-6
|
Authors |
William Okech, Catherine K. Kuo |
Editors |
Paul W. Ackermann, David A. Hart |
Abstract |
Adult tendons fail to regenerate normal tissue after injury, and instead form dysfunctional scar tissue with abnormal mechanical properties. Surgical repair with grafts is the current standard to treat injuries, but faces significant limitations including pain and high rates of re-injury. To address this, we aim to regenerate new, normal tendons to replace dysfunctional tendons. A common approach to tendon tissue engineering is to design scaffolds and bioreactors based on adult tendon properties that can direct adult stem cell tenogenesis. Despite significant progress, advances have been limited due, in part, to a need for markers and potent induction cues. Our goal is to develop novel tendon tissue engineering approaches informed by embryonic tendon development. We are characterizing structure-property relationships of embryonic tendon to identify design parameters for three-dimensional scaffolds and bioreactor mechanical loading systems to direct adult stem cell tenogenesis. We will review studies in which we quantified changes in the mechanical and biochemical properties of tendon during embryonic development and elucidated specific mechanisms of functional property elaboration. We then examined the effects of these mechanical and biochemical factors on embryonic tendon cell behavior. Using custom-designed bioreactors, we also examined the effects of dynamic mechanical loading and growth factor treatment on embryonic tendon cells. Our findings have established cues to induce tenogenesis as well as metrics to evaluate differentiation. We finish by discussing how we have evaluated the tenogenic differentiation potential of adult stem cells by comparing their responses to that of embryonic tendon cells in these culture systems. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 25 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 32% |
Student > Bachelor | 4 | 16% |
Student > Doctoral Student | 3 | 12% |
Lecturer | 1 | 4% |
Lecturer > Senior Lecturer | 1 | 4% |
Other | 4 | 16% |
Unknown | 4 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 7 | 28% |
Medicine and Dentistry | 5 | 20% |
Biochemistry, Genetics and Molecular Biology | 3 | 12% |
Materials Science | 2 | 8% |
Chemical Engineering | 1 | 4% |
Other | 3 | 12% |
Unknown | 4 | 16% |