Chapter title |
Uptake and Permeability Studies to Delineate the Role of Efflux Transporters.
|
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Chapter number | 5 |
Book title |
Cancer Drug Resistance
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3347-1_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3345-7, 978-1-4939-3347-1
|
Authors |
Ramya Krishna Vadlapatla, Dhananjay Pal, Ashim K. Mitra Ph.D., Ashim K. Mitra |
Editors |
José Rueff, António Sebastião Rodrigues |
Abstract |
Chemotherapy is one of the major therapeutic interventions in oncology. Despite numerous advances and intensive research, a large number of patients acquire multidrug resistance (MDR) and no longer respond to chemotherapy. Efflux transporters play a predominant role in mediating MDR. Cellular accumulation (uptake) and permeability studies serve as invaluable methods to detect drug efflux/transport mechanism. These methods are generally performed on transfected cells (e.g., MDCKII-MDR1, MDCKII-MRP2, and MDCKII-BCRP) or cells expressing high amount of intrinsic efflux transporters (Caco-2) utilizing specific inhibitors as positive controls. This chapter presents a method of performing uptake and permeability studies, including the preparation of various buffers required for the study. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 50% |
Professor | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 50% |
Unknown | 1 | 50% |