Chapter title |
Enhanced DNA Profiling of the Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome-Targeted Pre-amplification and Next Generation Y-STR Amplification Systems.
|
---|---|
Chapter number | 15 |
Book title |
Forensic DNA Typing Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3597-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3595-6, 978-1-4939-3597-0
|
Authors |
Erin K. Hanson Ph.D., Jack Ballantyne Ph.D., Erin K. Hanson, Jack Ballantyne |
Editors |
William Goodwin |
Abstract |
In some cases of sexual assault the victim may not report the assault for several days after the incident due to various factors. The ability to obtain an autosomal STR profile of the semen donor from a living victim rapidly diminishes as the post-coital interval is extended due to the presence of only a small amount of male DNA amidst an overwhelming amount of female DNA. Previously, we have utilized various technological tools to overcome the limitations of male DNA profiling in extended interval post-coital samples including the use of Y-chromosome STR profiling, cervical sample, and post-PCR purification permitting the recovery of Y-STR profiles of the male DNA from samples collected 5-6 days after intercourse. Despite this success, the reproductive biology literature reports the presence of spermatozoa in the human cervix up to 7-10 days post-coitus. Therefore, novel and improved methods for recovery of male profiles in extended interval post-coital samples were required. Here, we describe enhanced strategies, including Y-chromosome-targeted pre-amplification and next generation Y-STR amplification kits, that have resulted in the ability to obtain probative male profiles from samples collected 6-9 days after intercourse. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Portugal | 1 | 10% |
Unknown | 9 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 20% |
Student > Doctoral Student | 1 | 10% |
Librarian | 1 | 10% |
Student > Master | 1 | 10% |
Student > Bachelor | 1 | 10% |
Other | 0 | 0% |
Unknown | 4 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 10% |
Agricultural and Biological Sciences | 1 | 10% |
Psychology | 1 | 10% |
Social Sciences | 1 | 10% |
Medicine and Dentistry | 1 | 10% |
Other | 0 | 0% |
Unknown | 5 | 50% |