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Forensic DNA Typing Protocols

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Cover of 'Forensic DNA Typing Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Collection of Samples for DNA Analysis.
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    Chapter 2 Body Fluid Identification Using mRNA Profiling.
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    Chapter 3 mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification.
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    Chapter 4 Preservation of and DNA Extraction from Muscle Tissue.
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    Chapter 5 DNA Extraction: Organic and Solid-Phase.
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    Chapter 6 Extraction of DNA from Skeletal Remains.
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    Chapter 7 Extraction of DNA from Human Skeletal Material.
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    Chapter 8 The Development and Use of Internal Amplification Controls (IACs) with DNA Profiling Kits for Forensic DNA Analysis.
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    Chapter 9 NucleoSpin(®) XS Columns for DNA Concentration and Clean-Up.
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    Chapter 10 Purification of PCR Products to Improve STR Profiles.
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    Chapter 11 Analysis of 30 Biallelic INDEL Markers Using the Investigator DIPplex(®) Kit.
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    Chapter 12 Analysis of Mitochondrial Control Region Using Sanger Sequencing.
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    Chapter 13 Forensic DNA Typing Protocols
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    Chapter 14 In-Solution Hybridization for the Targeted Enrichment of the Whole Mitochondrial Genome.
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    Chapter 15 Enhanced DNA Profiling of the Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome-Targeted Pre-amplification and Next Generation Y-STR Amplification Systems.
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    Chapter 16 Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y-STRs).
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    Chapter 17 A Practical Guide to the HIrisPlex System: Simultaneous Prediction of Eye and Hair Color from DNA.
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    Chapter 18 Inference of Ancestry in Forensic Analysis I: Autosomal Ancestry-Informative Marker Sets.
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    Chapter 19 Inference of Ancestry in Forensic Analysis II: Analysis of Genetic Data.
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    Chapter 20 Species Determination: The Role and Use of the Cytochrome b Gene.
Attention for Chapter 5: DNA Extraction: Organic and Solid-Phase.
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Chapter title
DNA Extraction: Organic and Solid-Phase.
Chapter number 5
Book title
Forensic DNA Typing Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3597-0_5
Pubmed ID
Book ISBNs
978-1-4939-3595-6, 978-1-4939-3597-0
Authors

Wafa Altayari

Editors

William Goodwin

Abstract

DNA extraction remains a critical step in DNA profiling of biological material recovered from scenes of crime. In the forensic community several methods have gained popularity, including Chelex(®), organic extraction, and solid-phase extraction. While some laboratories streamlined their processes and only use one method we have retained several methods and continue to use these for different sample types. In this chapter we present three methods that have been used for several years in our laboratory.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 3%
Unknown 31 97%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 22%
Student > Master 2 6%
Lecturer 1 3%
Student > Ph. D. Student 1 3%
Student > Doctoral Student 1 3%
Other 2 6%
Unknown 18 56%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 25%
Agricultural and Biological Sciences 2 6%
Chemistry 2 6%
Psychology 1 3%
Nursing and Health Professions 1 3%
Other 0 0%
Unknown 18 56%