Chapter title |
NucleoSpin(®) XS Columns for DNA Concentration and Clean-Up.
|
---|---|
Chapter number | 9 |
Book title |
Forensic DNA Typing Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3597-0_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3595-6, 978-1-4939-3597-0
|
Authors |
William R. Hudlow |
Editors |
William Goodwin |
Abstract |
The phenol-chloroform (organic) extraction method continues to be a preferred method for extraction of DNA from forensic evidence samples that may contain low quantities of DNA and polymerase chain reaction (PCR) inhibitors. The aqueous extracts from the organic extraction of DNA require subsequent concentration and cleanup, which has traditionally been performed with microdialysis filter units, including the Centricon(®) and Microcon(®) centrifugal filter devices. Here, we describe the use of the NucleoSpin(®) XS silica columns as an alternative for the concentration and purification of the aqueous extracts from the organic extraction and for the removal of PCR inhibitors from existing DNA extracts. |
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