Chapter title |
Auditory and Vestibular Research
|
---|---|
Chapter number | 2 |
Book title |
Auditory and Vestibular Research
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3615-1_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3613-7, 978-1-4939-3615-1
|
Authors |
Stoller, Michelle L, Fekete, Donna M, Michelle L. Stoller, Donna M. Fekete |
Editors |
Bernd Sokolowski |
Abstract |
The avian embryo has a well-documented history as a model system for the study of neurogenesis, morphogenesis, and cell fate specification. This includes studies of the chicken inner ear that employ in ovo electroporation, in conjunction with the Tol2 system, to yield robust long-term transgene expression. Capitalizing on the success of this delivery method, we describe a modified version of the Tol2 expression vector that readily accepts the insertion of a microRNA-encoding artificial intron. This offers a strategy to investigate the possible roles of different candidate microRNAs in ear development by overexpression. Here, we describe the general design of this modified vector and the electroporation procedure. This approach is expected to facilitate phenotypic screening of candidate miRNAs to explore their bioactivity in vivo. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Postgraduate | 1 | 33% |
Other | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 1 | 33% |
Unknown | 2 | 67% |