Chapter title |
A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells
|
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Chapter number | 28 |
Book title |
Auditory and Vestibular Research
|
Published in |
Methods in molecular biology, June 2016
|
DOI | 10.1007/978-1-4939-3615-1_28 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3613-7, 978-1-4939-3615-1
|
Authors |
Lei Song, Joseph Santos-Sacchi |
Editors |
Bernd Sokolowski |
Abstract |
Nonlinear capacitance (NLC) measures are often used as surrogate measures of outer hair cell (OHC) electromotility (eM), since the two are commonly thought to share many biophysical features. The measurement of NLC is simpler than direct measurements of eM and, therefore, many investigators have adopted it. A standard patch-clamp hardware configuration is sufficient for recording NLC, given the proper software interface. Thus, the approach is cost effective. We use the software jClamp since it is tailored to capacitance measurement. Here we detail steps that we use to measure NLC. The walk through includes isolation of guinea pig OHCs, building voltage commands, recording, and analysis. |
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