| Chapter title |
Auditory and Vestibular Research
|
|---|---|
| Chapter number | 30 |
| Book title |
Auditory and Vestibular Research
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3615-1_30 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3613-7, 978-1-4939-3615-1
|
| Authors |
Sturm, Joshua J, Nguyen, Tuan, Kandler, Karl, Joshua J. Sturm, Tuan Nguyen, Karl Kandler |
| Editors |
Bernd Sokolowski |
| Abstract |
Photostimulation of neurons with caged glutamate is a viable tool for mapping the strength and spatial distribution of synaptic networks in living brain slices. In photostimulation experiments, synaptic connectivity is assessed by eliciting action potentials in putative presynaptic neurons via focal photolysis of caged glutamate, while measuring postsynaptic responses via intracellular recordings. Two approaches are commonly used for delivering light to small, defined areas in the slice preparation; an optical fiber-based method and a laser-scanning-based method. In this chapter, we outline the technical bases for using photostimulation of caged glutamate to map synaptic circuits, and discuss the advantages and disadvantages of using fiber-based vs. laser-based systems. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 29% |
| Student > Bachelor | 2 | 29% |
| Professor | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 4 | 57% |
| Agricultural and Biological Sciences | 1 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Unknown | 1 | 14% |