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Mendeley readers
Chapter title |
High-Throughput Arginylation Assay in Microplate Format.
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Chapter number | 11 |
Book title |
Protein Arginylation
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2935-1_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2934-4, 978-1-4939-2935-1
|
Authors |
Sougata Saha, Junling Wang, Anna S. Kashina |
Editors |
Anna S. Kashina |
Abstract |
Here we describe the biochemical assay for ATE1-mediated arginylation in microplate format, which can be applied to high-throughput screens for identification of small-molecule inhibitors and activators of ATE1, high-volume analysis of ATE1 substrates, and other similar applications. Originally, we have applied this screen to a library of 3280 compounds and identified two compounds which specifically affect ATE1-regulated processes in vitro and in vivo. The assay is based on in vitro ATE1-mediated arginylation of beta-actin's N-terminal peptide, but it can also be applied using other ATE1 substrates. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 50% |
Lecturer | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 50% |
Agricultural and Biological Sciences | 1 | 50% |