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Mendeley readers
Chapter title |
Assaying the Posttranslational Arginylation of Proteins in Cultured Cells.
|
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Chapter number | 7 |
Book title |
Protein Arginylation
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2935-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2934-4, 978-1-4939-2935-1
|
Authors |
Mauricio R. Galiano, Marta E. Hallak |
Editors |
Anna S. Kashina |
Abstract |
To evaluate the posttranslational arginylation of proteins in vivo, we describe a protocol for studying the (14)C-Arg incorporation into proteins of cells in culture. The conditions determined for this particular modification contemplate both the biochemical requirements of the enzyme ATE1 and the adjustments that allowed the discrimination between posttranslational arginylation of proteins and de novo synthesis. These conditions are applicable for different cell lines or primary cultures, representing an optimal procedure for the identification and the validation of putative ATE1 substrates. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Lecturer | 1 | 50% |
Lecturer > Senior Lecturer | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 50% |
Agricultural and Biological Sciences | 1 | 50% |