You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Timeline
Mendeley readers
Chapter title |
Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles.
|
---|---|
Chapter number | 15 |
Book title |
Protein Arginylation
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2935-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2934-4, 978-1-4939-2935-1
|
Authors |
Anne M. Wagner, John B. Warner, Haviva E. Garrett, Christopher R. Walters, E. James Petersson |
Editors |
Anna S. Kashina |
Abstract |
The E. coli aminoacyl transferase (AaT) can be used to transfer a variety of unnatural amino acids, including those with azide or alkyne groups, to the α-amine of a protein with an N-terminal Lys or Arg. Subsequent functionalization through either copper-catalyzed or strain-promoted click reactions can be used to label the protein with fluorophores or biotin. This method can be used to directly detect AaT substrates or in a two-step protocol to detect substrates of the mammalian ATE1 transferase. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 50% |
Lecturer | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 100% |