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Mendeley readers
Chapter title |
Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays.
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Chapter number | 9 |
Book title |
Protein Arginylation
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2935-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2934-4, 978-1-4939-2935-1
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Authors |
Junling Wang, Anna S. Kashina |
Editors |
Anna S. Kashina |
Abstract |
Here we describe the procedure for expression and purification of recombinant ATE1 from E. coli. This method is easy and convenient and can result in one-step isolation of milligram amounts of soluble enzymatically active ATE1 at nearly 99 % purity. We also describe a procedure for expression and purification of E. coli Arg-tRNA synthetase essential for the arginylation assays described in the next two chapters. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 25% |
Student > Ph. D. Student | 1 | 25% |
Professor > Associate Professor | 1 | 25% |
Lecturer | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Chemical Engineering | 2 | 50% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Agricultural and Biological Sciences | 1 | 25% |