Chapter title |
ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts.
|
---|---|
Chapter number | 3 |
Book title |
Advanced Technologies for Protein Complex Production and Characterization
|
Published in |
Advances in experimental medicine and biology, May 2016
|
DOI | 10.1007/978-3-319-27216-0_3 |
Pubmed ID | |
Book ISBNs |
978-3-31-927214-6, 978-3-31-927216-0
|
Authors |
Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel, Imre Berger, Nie, Yan, Chaillet, Maxime, Becke, Christian, Haffke, Matthias, Pelosse, Martin, Fitzgerald, Daniel, Collinson, Ian, Schaffitzel, Christiane, Berger, Imre |
Editors |
M. Cristina Vega |
Abstract |
Multicomponent biological systems perform a wide variety of functions and are crucially important for a broad range of critical health and disease states. A multitude of applications in contemporary molecular and synthetic biology rely on efficient, robust and flexible methods to assemble multicomponent DNA circuits as a prerequisite to recapitulate such biological systems in vitro and in vivo. Numerous functionalities need to be combined to allow for the controlled realization of information encoded in a defined DNA circuit. Much of biological function in cells is catalyzed by multiprotein machines typically made up of many subunits. Provision of these multiprotein complexes in the test-tube is a vital prerequisite to study their structure and function, to understand biology and to develop intervention strategies to correct malfunction in disease states. ACEMBL is a technology concept that specifically addresses the requirements of multicomponent DNA assembly into multigene constructs, for gene delivery and the production of multiprotein complexes in high-throughput. ACEMBL is applicable to prokaryotic and eukaryotic expression hosts, to accelerate basic and applied research and development. The ACEMBL concept, reagents, protocols and its potential are reviewed in this contribution. |
X Demographics
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Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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China | 1 | 6% |
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Demographic breakdown
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Researcher | 5 | 28% |
Student > Ph. D. Student | 3 | 17% |
Lecturer > Senior Lecturer | 2 | 11% |
Student > Bachelor | 2 | 11% |
Other | 1 | 6% |
Other | 2 | 11% |
Unknown | 3 | 17% |
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Immunology and Microbiology | 1 | 6% |
Economics, Econometrics and Finance | 1 | 6% |
Earth and Planetary Sciences | 1 | 6% |
Other | 0 | 0% |
Unknown | 4 | 22% |