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Advanced Technologies for Protein Complex Production and Characterization

Overview of attention for book
Advanced Technologies for Protein Complex Production and Characterization
Springer International Publishing

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Protein Complex Production from the Drug Discovery Standpoint.
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    Chapter 2 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 3 ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts.
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    Chapter 4 Complex Reconstitution and Characterization by Combining Co-expression Techniques in Escherichia coli with High-Throughput.
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    Chapter 5 Membrane Protein Production in E. coli for Applications in Drug Discovery.
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    Chapter 6 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 7 A Bacillus megaterium System for the Production of Recombinant Proteins and Protein Complexes.
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    Chapter 8 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 9 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 10 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 11 Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes.
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    Chapter 12 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 13 The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics.
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    Chapter 14 Fundamentals of Expression in Mammalian Cells.
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    Chapter 15 Assembling Multi-subunit Complexes Using Mammalian Expression.
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    Chapter 16 Microalgae as Solar-Powered Protein Factories.
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    Chapter 17 Strategies and Methodologies for the Co-expression of Multiple Proteins in Plants.
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    Chapter 18 Transient Expression Systems in Plants: Potentialities and Constraints.
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    Chapter 19 Complex Reconstitution from Individual Protein Modules.
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    Chapter 20 Structural Reconstruction of Protein-Protein Complexes Involved in Intracellular Signaling.
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    Chapter 21 The Use of Small-Angle Scattering for the Characterization of Multi Subunit Complexes.
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    Chapter 22 Application of Nuclear Magnetic Resonance and Hybrid Methods to Structure Determination of Complex Systems.
Attention for Chapter 13: The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics.
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (66th percentile)
  • High Attention Score compared to outputs of the same age and source (85th percentile)

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Chapter title
The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics.
Chapter number 13
Book title
Advanced Technologies for Protein Complex Production and Characterization
Published in
Advances in experimental medicine and biology, May 2016
DOI 10.1007/978-3-319-27216-0_13
Pubmed ID
Book ISBNs
978-3-31-927214-6, 978-3-31-927216-0
Authors

Duygu Sari, Kapil Gupta, Deepak Balaji Thimiri Govinda Raj, Alice Aubert, Petra Drncová, Frederic Garzoni, Daniel Fitzgerald, Imre Berger, Sari, Duygu, Gupta, Kapil, Raj, Deepak Balaji Thimiri Govinda, Aubert, Alice, Drncová, Petra, Garzoni, Frederic, Fitzgerald, Daniel, Berger, Imre

Editors

M. Cristina Vega

Abstract

Multiprotein complexes regulate most if not all cellular functions. Elucidating the structure and function of these complex cellular machines is essential for understanding biology. Moreover, multiprotein complexes by themselves constitute powerful reagents as biologics for the prevention and treatment of human diseases. Recombinant production by the baculovirus/insect cell expression system is particularly useful for expressing proteins of eukaryotic origin and their complexes. MultiBac, an advanced baculovirus/insect cell system, has been widely adopted in the last decade to produce multiprotein complexes with many subunits that were hitherto inaccessible, for academic and industrial research and development. The MultiBac system, its development and numerous applications are presented. Future opportunities for utilizing MultiBac to catalyze discovery are outlined.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 104 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 <1%
China 1 <1%
Canada 1 <1%
Unknown 101 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 19 18%
Student > Master 13 13%
Student > Bachelor 13 13%
Student > Ph. D. Student 11 11%
Student > Postgraduate 6 6%
Other 15 14%
Unknown 27 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 32 31%
Agricultural and Biological Sciences 22 21%
Chemistry 4 4%
Medicine and Dentistry 3 3%
Pharmacology, Toxicology and Pharmaceutical Science 2 2%
Other 9 9%
Unknown 32 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 December 2022.
All research outputs
#8,175,364
of 26,363,900 outputs
Outputs from Advances in experimental medicine and biology
#1,253
of 5,273 outputs
Outputs of similar age
#108,095
of 326,365 outputs
Outputs of similar age from Advances in experimental medicine and biology
#19
of 130 outputs
Altmetric has tracked 26,363,900 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 5,273 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.3. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 326,365 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 130 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.