Chapter title |
Fundamentals of Expression in Mammalian Cells.
|
---|---|
Chapter number | 14 |
Book title |
Advanced Technologies for Protein Complex Production and Characterization
|
Published in |
Advances in experimental medicine and biology, May 2016
|
DOI | 10.1007/978-3-319-27216-0_14 |
Pubmed ID | |
Book ISBNs |
978-3-31-927214-6, 978-3-31-927216-0
|
Authors |
Michael R. Dyson |
Editors |
M. Cristina Vega |
Abstract |
Expression of proteins in mammalian cells is a key technology important for many functional studies on human and higher eukaryotic genes. Studies include the mapping of protein interactions, solving protein structure by crystallization and X-ray diffraction or solution phase NMR and the generation of antibodies to enable a range of studies to be performed including protein detection in vivo. In addition the production of therapeutic proteins and antibodies, now a multi billion dollar industry, has driven major advances in cell line engineering for the production of grams per liter of active proteins and antibodies. Here the key factors that need to be considered for successful expression in HEK293 and CHO cells are reviewed including host cells, expression vector design, transient transfection methods, stable cell line generation and cultivation conditions. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | <1% |
Unknown | 116 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 22 | 19% |
Student > Ph. D. Student | 19 | 16% |
Student > Bachelor | 18 | 15% |
Researcher | 13 | 11% |
Other | 4 | 3% |
Other | 7 | 6% |
Unknown | 34 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 39 | 33% |
Agricultural and Biological Sciences | 18 | 15% |
Immunology and Microbiology | 4 | 3% |
Chemistry | 4 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 3% |
Other | 13 | 11% |
Unknown | 36 | 31% |