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Advanced Technologies for Protein Complex Production and Characterization

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Cover of 'Advanced Technologies for Protein Complex Production and Characterization'

Table of Contents

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    Book Overview
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    Chapter 1 Protein Complex Production from the Drug Discovery Standpoint.
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    Chapter 2 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 3 ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts.
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    Chapter 4 Complex Reconstitution and Characterization by Combining Co-expression Techniques in Escherichia coli with High-Throughput.
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    Chapter 5 Membrane Protein Production in E. coli for Applications in Drug Discovery.
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    Chapter 6 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 7 A Bacillus megaterium System for the Production of Recombinant Proteins and Protein Complexes.
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    Chapter 8 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 9 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 10 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 11 Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes.
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    Chapter 12 Advanced Technologies for Protein Complex Production and Characterization
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    Chapter 13 The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics.
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    Chapter 14 Fundamentals of Expression in Mammalian Cells.
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    Chapter 15 Assembling Multi-subunit Complexes Using Mammalian Expression.
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    Chapter 16 Microalgae as Solar-Powered Protein Factories.
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    Chapter 17 Strategies and Methodologies for the Co-expression of Multiple Proteins in Plants.
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    Chapter 18 Transient Expression Systems in Plants: Potentialities and Constraints.
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    Chapter 19 Complex Reconstitution from Individual Protein Modules.
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    Chapter 20 Structural Reconstruction of Protein-Protein Complexes Involved in Intracellular Signaling.
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    Chapter 21 The Use of Small-Angle Scattering for the Characterization of Multi Subunit Complexes.
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    Chapter 22 Application of Nuclear Magnetic Resonance and Hybrid Methods to Structure Determination of Complex Systems.
Attention for Chapter 17: Strategies and Methodologies for the Co-expression of Multiple Proteins in Plants.
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Chapter title
Strategies and Methodologies for the Co-expression of Multiple Proteins in Plants.
Chapter number 17
Book title
Advanced Technologies for Protein Complex Production and Characterization
Published in
Advances in experimental medicine and biology, May 2016
DOI 10.1007/978-3-319-27216-0_17
Pubmed ID
Book ISBNs
978-3-31-927214-6, 978-3-31-927216-0
Authors

Albert Ferrer, Monserrat Arró, David Manzano, Teresa Altabella

Editors

M. Cristina Vega

Abstract

The first transgenes were introduced in a plant genome more than 30 years ago. Since then, the capabilities of the plant scientific community to engineer the genome of plants have progressed at an unparalleled speed. Plant genetic engineering has become a central technology that has dramatically incremented our basic knowledge of plant biology and has enabled the translation of this knowledge into a number of increasingly complex and sophisticated biotechnological applications, which in most cases rely on the simultaneous co-expression of multiple recombinant proteins from different origins. To meet the new challenges of modern plant biotechnology, the plant scientific community has developed a vast arsenal of innovative molecular tools and genome engineering strategies. In this chapter we review a variety of tools, technologies, and strategies developed to transfer and simultaneously co-express multiple transgenes and proteins in a plant host. Their potential advantages, disadvantages, and future prospects are also discussed.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 17%
Researcher 4 17%
Student > Doctoral Student 3 13%
Student > Ph. D. Student 3 13%
Student > Bachelor 2 9%
Other 3 13%
Unknown 4 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 48%
Biochemistry, Genetics and Molecular Biology 6 26%
Medicine and Dentistry 1 4%
Engineering 1 4%
Unknown 4 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 May 2016.
All research outputs
#20,326,948
of 22,870,727 outputs
Outputs from Advances in experimental medicine and biology
#3,970
of 4,951 outputs
Outputs of similar age
#263,487
of 309,583 outputs
Outputs of similar age from Advances in experimental medicine and biology
#106
of 131 outputs
Altmetric has tracked 22,870,727 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,951 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 131 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.