Chapter title |
Advanced Technologies for Protein Complex Production and Characterization
|
---|---|
Chapter number | 9 |
Book title |
Advanced Technologies for Protein Complex Production and Characterization
|
Published in |
Advances in experimental medicine and biology, May 2016
|
DOI | 10.1007/978-3-319-27216-0_9 |
Pubmed ID | |
Book ISBNs |
978-3-31-927214-6, 978-3-31-927216-0
|
Authors |
Fernández, Francisco J, López-Estepa, Miguel, Querol-García, Javier, Vega, M Cristina, Francisco J. Fernández, Miguel López-Estepa, Javier Querol-García, M. Cristina Vega |
Editors |
M. Cristina Vega |
Abstract |
Protein complexes can be produced in multimilligram quantities using nonmethylotrophic and methylotrophic yeasts such as Saccharomyces cerevisiae and Komagataella (Pichia) pastoris. Yeasts have distinct advantages as hosts for recombinant protein production owing to their cost efficiency, ease of cultivation and genetic manipulation, fast growth rates, capacity to introduce post-translational modifications, and high protein productivity (yield) of correctly folded protein products. Despite those advantages, yeasts have surprisingly lagged behind other eukaryotic hosts in their use for the production of multisubunit complexes. As our knowledge of the metabolic and genomic bottlenecks that yeast microorganisms face when overexpressing foreign proteins expands, new possibilities emerge for successfully engineering yeasts as superb expression hosts. In this chapter, we describe the current state of the art and discuss future possibilities for the development of yeast-based systems for the production of protein complexes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 4% |
Unknown | 24 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 20% |
Student > Bachelor | 5 | 20% |
Student > Master | 4 | 16% |
Student > Ph. D. Student | 3 | 12% |
Other | 2 | 8% |
Other | 4 | 16% |
Unknown | 2 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 28% |
Biochemistry, Genetics and Molecular Biology | 6 | 24% |
Chemical Engineering | 3 | 12% |
Linguistics | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Other | 5 | 20% |
Unknown | 2 | 8% |