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Dendritic Cell Protocols

Overview of attention for book
Cover of 'Dendritic Cell Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Review of Mouse and Human Dendritic Cell Subsets.
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    Chapter 2 In Vitro Generation of Human XCR1(+) Dendritic Cells from CD34(+) Hematopoietic Progenitors.
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    Chapter 3 Derivation and Utilization of Functional CD8(+) Dendritic Cell Lines.
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    Chapter 4 Dendritic Cell Protocols
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    Chapter 5 The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and Their In Vitro Equivalents.
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    Chapter 6 Dendritic Cell Subset Purification from Human Tonsils and Lymph Nodes.
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    Chapter 7 Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men.
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    Chapter 8 Isolation of Human Skin Dendritic Cell Subsets.
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    Chapter 9 Isolation of Mouse Dendritic Cell Subsets and Macrophages from the Skin.
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    Chapter 10 Isolation of Conventional Dendritic Cells from Mouse Lungs.
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    Chapter 11 Purification of Human Dendritic Cell Subsets from Peripheral Blood.
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    Chapter 12 Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues.
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    Chapter 13 Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay.
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    Chapter 14 Dendritic Cell Protocols
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    Chapter 15 Dendritic Cell Protocols
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    Chapter 16 Dendritic Cell Protocols
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    Chapter 17 Dendritic Cell Protocols
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    Chapter 18 Dendritic Cell Protocols
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    Chapter 19 Dendritic Cell Protocols
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    Chapter 20 Dendritic Cell Protocols
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    Chapter 21 Dendritic Cell Protocols
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    Chapter 22 Generation of Humanized Mice for Analysis of Human Dendritic Cells.
Attention for Chapter 4: Dendritic Cell Protocols
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Chapter title
Dendritic Cell Protocols
Chapter number 4
Book title
Dendritic Cell Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3606-9_4
Pubmed ID
27142008
Book ISBNs
978-1-4939-3604-5, 978-1-4939-3606-9
Authors

Onai, Nobuyuki, Ohteki, Toshiaki, Nobuyuki Onai, Toshiaki Ohteki

Editors

Elodie Segura, Nobuyuki Onai

Abstract

Dendritic cells (DCs) comprise two major subsets, conventional DC (cDC) and plasmacytoid DC (pDC) in the steady-state lymphoid organ. These cells have a short half-life and therefore, require continuous generation from hematopoietic stem cells and progenitor cells. Recently, we identified DC-restricted progenitors called common DC progenitors (CDPs) in the bone marrow of mouse. The CDPs can be isolated from mouse bone marrow based on the hematopoietic cytokine receptors, such as Flt3 (Fms-related tyrosine kinase 3) (CD135), c-kit (CD117), M-CSF (macrophage colony-stimulating factor) receptor (CD115), and IL-7 (interleukin-7) receptor-α (CD127). The CDPs comprise of two progenitors, CD115(+) CDPs and CD115(-) CDPs, and give rise to only DC subsets in both in vitro and in vivo. The former CDPs are the main source of cDC, while the later CDPs are the main source of pDC in vivo. Here, we provide a protocol for the isolation of dendritic cell progenitor and bone marrow progenitor cells from mouse.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 25%
Professor > Associate Professor 2 25%
Professor 1 13%
Unspecified 1 13%
Researcher 1 13%
Other 1 13%
Readers by discipline Count As %
Immunology and Microbiology 3 38%
Biochemistry, Genetics and Molecular Biology 1 13%
Unspecified 1 13%
Agricultural and Biological Sciences 1 13%
Medicine and Dentistry 1 13%
Other 0 0%
Unknown 1 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2016.
All research outputs
#18,455,405
of 22,867,327 outputs
Outputs from Methods in molecular biology
#7,923
of 13,128 outputs
Outputs of similar age
#284,496
of 393,648 outputs
Outputs of similar age from Methods in molecular biology
#846
of 1,470 outputs
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So far Altmetric has tracked 13,128 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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