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Dendritic Cell Protocols

Overview of attention for book
Cover of 'Dendritic Cell Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Review of Mouse and Human Dendritic Cell Subsets.
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    Chapter 2 In Vitro Generation of Human XCR1(+) Dendritic Cells from CD34(+) Hematopoietic Progenitors.
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    Chapter 3 Derivation and Utilization of Functional CD8(+) Dendritic Cell Lines.
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    Chapter 4 Dendritic Cell Protocols
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    Chapter 5 The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and Their In Vitro Equivalents.
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    Chapter 6 Dendritic Cell Subset Purification from Human Tonsils and Lymph Nodes.
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    Chapter 7 Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men.
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    Chapter 8 Isolation of Human Skin Dendritic Cell Subsets.
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    Chapter 9 Isolation of Mouse Dendritic Cell Subsets and Macrophages from the Skin.
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    Chapter 10 Isolation of Conventional Dendritic Cells from Mouse Lungs.
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    Chapter 11 Purification of Human Dendritic Cell Subsets from Peripheral Blood.
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    Chapter 12 Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues.
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    Chapter 13 Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay.
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    Chapter 14 Dendritic Cell Protocols
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    Chapter 15 Dendritic Cell Protocols
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    Chapter 16 Dendritic Cell Protocols
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    Chapter 17 Dendritic Cell Protocols
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    Chapter 18 Dendritic Cell Protocols
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    Chapter 19 Dendritic Cell Protocols
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    Chapter 20 Dendritic Cell Protocols
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    Chapter 21 Dendritic Cell Protocols
  23. Altmetric Badge
    Chapter 22 Generation of Humanized Mice for Analysis of Human Dendritic Cells.
Attention for Chapter 20: Dendritic Cell Protocols
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Chapter title
Dendritic Cell Protocols
Chapter number 20
Book title
Dendritic Cell Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3606-9_20
Pubmed ID
Book ISBNs
978-1-4939-3604-5, 978-1-4939-3606-9
Authors

Tetlak, Piotr, Ruedl, Christiane, Piotr Tetlak, Christiane Ruedl

Editors

Elodie Segura, Nobuyuki Onai

Abstract

The Clec9A-diphtheria toxin receptor (DTR) transgenic mouse strain provides a robust animal model to study the function of lymphoid organ-resident CD8(+) dendritic cells (DCs) and nonlymphoid organ-specific CD103(+) DCs in infectioous diseases and inflammation. Here we describe some basic protocols for CD8(+)/CD103(+) DC isolation, for their in vivo depletion, and for their characterization by multi-color flow cytometry analysis. As an example for in vivo functional characterization of this DC subset, we present here the experimental cerebral malaria model. Furthermore, we illustrate advantages and pitfalls of the Clec9A-DTR system.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 20%
Student > Doctoral Student 1 10%
Student > Ph. D. Student 1 10%
Student > Master 1 10%
Researcher 1 10%
Other 0 0%
Unknown 4 40%
Readers by discipline Count As %
Immunology and Microbiology 3 30%
Medicine and Dentistry 2 20%
Nursing and Health Professions 1 10%
Unknown 4 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2016.
All research outputs
#14,848,594
of 22,867,327 outputs
Outputs from Methods in molecular biology
#4,700
of 13,128 outputs
Outputs of similar age
#218,962
of 393,648 outputs
Outputs of similar age from Methods in molecular biology
#469
of 1,470 outputs
Altmetric has tracked 22,867,327 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,128 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,648 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.