Report for: Optogenetics

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Chapter title	Optogenetics
Chapter number	26
Book title	Optogenetics
Published in	Methods in molecular biology, January 2016
DOI	10.1007/978-1-4939-3512-3_26
Pubmed ID	26965137
Book ISBNs	978-1-4939-3510-9, 978-1-4939-3512-3
Authors	Hsiao, Po-Yen, Wu, Ming-Chin, Lin, Yen-Yin, Fu, Chein-Chung, Chiang, Ann-Shyn, Po-Yen Hsiao, Ming-Chin Wu, Yen-Yin Lin, Chein-Chung Fu, Ann-Shyn Chiang
Editors	Arash Kianianmomeni
Abstract	Activating selected neurons elicits specific behaviors in Drosophila adults. By combining optogenetics and laser-tracking techniques, we have recently developed an automated laser-tracking and optogenetic manipulation system (ALTOMS) for studying how brain circuits orchestrate complex behaviors. The established ALTOMS can independently target three lasers (473-nm blue laser, 593.5-nm yellow laser, and 1064-nm infrared laser) on any specified body part of two freely moving flies. Triggering light-sensitive proteins in real time, the blue laser and yellow laser can respectively activate and inhibit target neurons in artificial transgenic flies. Since infrared light is invisible to flies, we use the 1064-nm laser as an aversive stimulus in operant learning without perturbing visual inputs. Herein, we provide a detailed protocol for the construction of ALTOMS and optogenetic manipulation of target neurons in Drosophila adults during social interactions.

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Geographical breakdown

Country	Count	As %
United States	1	100%

Demographic breakdown

Type	Count	As %
Members of the public	1	100%

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The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	6	100%

Demographic breakdown

Readers by professional status	Count	As %
Student > Ph. D. Student	2	33%
Student > Bachelor	1	17%
Researcher	1	17%
Student > Doctoral Student	1	17%
Unknown	1	17%

Readers by discipline	Count	As %
Engineering	2	33%
Neuroscience	2	33%
Immunology and Microbiology	1	17%
Unknown	1	17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 March 2016.

All research outputs

#19,015,492

of 23,577,654 outputs

Outputs from Methods in molecular biology

#8,199

of 13,410 outputs

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#288,141

of 396,838 outputs

Outputs of similar age from Methods in molecular biology

#873

of 1,472 outputs

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Optogenetics

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