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Mendeley readers
Chapter title |
Photo Control of Protein Function Using Photoactive Yellow Protein
|
---|---|
Chapter number | 6 |
Book title |
Optogenetics
|
Published in |
Methods in molecular biology, March 2016
|
DOI | 10.1007/978-1-4939-3512-3_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3510-9, 978-1-4939-3512-3
|
Authors |
Jakeb M. Reis, G. Andrew Woolley |
Editors |
Arash Kianianmomeni |
Abstract |
Photoswitchable proteins are becoming increasingly common tools for manipulating cellular processes with high spatial and temporal precision. Photoactive yellow protein (PYP) is a small, water-soluble protein that undergoes a blue light induced change in conformation. It can serve as a scaffold for designing new tools to manipulate biological processes, but with respect to other protein scaffolds it presents some technical challenges. Here, we present practical information on how to overcome these, including how to synthesize the PYP chromophore, how to express and purify PYP, and how to screen for desired activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 31% |
Student > Ph. D. Student | 2 | 15% |
Researcher | 2 | 15% |
Student > Master | 2 | 15% |
Unknown | 3 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 38% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Materials Science | 1 | 8% |
Neuroscience | 1 | 8% |
Chemistry | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 31% |