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Mendeley readers
Chapter title |
Study of CPP Mechanisms by Mass Spectrometry.
|
---|---|
Chapter number | 7 |
Book title |
Cell-Penetrating Peptides
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2806-4_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2805-7, 978-1-4939-2806-4
|
Authors |
Sandrine Sagan, Chérine Bechara, Fabienne Burlina |
Editors |
Ülo Langel |
Abstract |
Studying the mechanisms of entry of cell-penetrating peptides (CPPs) requires reliable methods to measure their cellular uptake efficiency, monitor their metabolic stability, and identify their intracellular localization. We describe here a protocol based on the direct detection of peptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which allows the absolute quantification of the intact internalized species and the analysis of their intracellular degradation. This protocol can be easily applied to the simultaneous quantification of different species, for example mixtures of CPPs. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Lecturer | 2 | 20% |
Researcher | 2 | 20% |
Lecturer > Senior Lecturer | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Other | 1 | 10% |
Other | 2 | 20% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 3 | 30% |
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Agricultural and Biological Sciences | 2 | 20% |
Medicine and Dentistry | 1 | 10% |
Veterinary Science and Veterinary Medicine | 1 | 10% |
Other | 0 | 0% |
Unknown | 1 | 10% |