Chapter title |
CPP-Based Delivery System for In Vivo Gene Delivery
|
---|---|
Chapter number | 22 |
Book title |
Cell-Penetrating Peptides
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2806-4_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2805-7, 978-1-4939-2806-4
|
Authors |
Kurrikoff, Kaido, Veiman, Kadi-Liis, Langel, Ülo, Kaido Kurrikoff, Kadi-Liis Veiman, Ülo Langel |
Editors |
Langel, Ülo |
Abstract |
The method presents most important steps in estimating a CPP-mediated reporter gene delivery in a mouse model. The method is applicable for administrating noncovalent CPP/pDNA complexes via i.v. injection and for analysis of luciferase levels in tissue homogenates. This method could be extended to analyze the delivery of different nucleic acid cargos with other types of delivery vectors. First, a simple method is presented for assessing the stability of complexes in blood after i.v. administration, based on quantitation of a fluorescently labeled nucleic acid. Secondly, a protocol is presented for assessing and analyzing luciferase levels in mouse organs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 50% |
Student > Ph. D. Student | 5 | 36% |
Lecturer | 1 | 7% |
Student > Master | 1 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 29% |
Agricultural and Biological Sciences | 3 | 21% |
Chemistry | 3 | 21% |
Neuroscience | 2 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Other | 0 | 0% |
Unknown | 1 | 7% |