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Chapter title |
Immuno-Electron Microscopy and Electron Microscopic In Situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries
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Chapter number | 12 |
Book title |
Drosophila Oogenesis
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2851-4_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2850-7, 978-1-4939-2851-4
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Authors |
Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano, Mikiko C. Siomi |
Abstract |
Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 38% |
Other | 2 | 25% |
Student > Bachelor | 1 | 13% |
Lecturer | 1 | 13% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 2 | 25% |
Agricultural and Biological Sciences | 2 | 25% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Unknown | 3 | 38% |