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Drosophila Oogenesis

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Cover of 'Drosophila Oogenesis'

Table of Contents

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    Book Overview
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    Chapter 1 Drosophila melanogaster Oogenesis: An Overview
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    Chapter 2 Basic Techniques in Drosophila Ovary Preparation
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    Chapter 3 Mosaic Analysis in the Drosophila melanogaster Ovary
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    Chapter 4 Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary.
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    Chapter 5 Culturing Drosophila Egg Chambers and Investigating Developmental Processes Through Live Imaging
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    Chapter 6 Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement
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    Chapter 7 Visualizing Microtubule Networks During Drosophila Oogenesis Using Fixed and Live Imaging
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    Chapter 8 Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers
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    Chapter 9 Single-Molecule RNA In Situ Hybridization (smFISH) and Immunofluorescence (IF) in the Drosophila Egg Chamber.
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    Chapter 10 Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries
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    Chapter 11 Ultrastructural Analysis of Drosophila Ovaries by Electron Microscopy
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    Chapter 12 Immuno-Electron Microscopy and Electron Microscopic In Situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries
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    Chapter 13 Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry
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    Chapter 14 Detection of Cell Death and Phagocytosis in the Drosophila Ovary
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    Chapter 15 Analysis of Cell Cycle Switches in Drosophila Oogenesis
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    Chapter 16 Drosophila Oogenesis
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    Chapter 17 Drosophila Oogenesis
Attention for Chapter 2: Basic Techniques in Drosophila Ovary Preparation
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Chapter title
Basic Techniques in Drosophila Ovary Preparation
Chapter number 2
Book title
Drosophila Oogenesis
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2851-4_2
Pubmed ID
Book ISBNs
978-1-4939-2850-7, 978-1-4939-2851-4
Authors

Letitia Thompson, Kristen Randolph, Amanda Norvell

Abstract

Drosophila melanogaster oogenesis has emerged as an excellent model system to study multiple aspects of eukaryotic cell biology. Ovarian tissue can easily be isolated and analyzed through microscopy or biochemical and molecular biology techniques. Here we describe the isolation of ovarian tissues, techniques to enrich for egg chambers at distinct developmental stages, preparation of protein and nucleic acid extracts, and preparation for microscopic analysis of fixed tissues.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 33%
Unspecified 1 17%
Professor > Associate Professor 1 17%
Student > Master 1 17%
Unknown 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 33%
Biochemistry, Genetics and Molecular Biology 2 33%
Unspecified 1 17%
Unknown 1 17%