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Drosophila Oogenesis

Overview of attention for book
Cover of 'Drosophila Oogenesis'

Table of Contents

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    Book Overview
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    Chapter 1 Drosophila melanogaster Oogenesis: An Overview
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    Chapter 2 Basic Techniques in Drosophila Ovary Preparation
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    Chapter 3 Mosaic Analysis in the Drosophila melanogaster Ovary
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    Chapter 4 Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary.
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    Chapter 5 Culturing Drosophila Egg Chambers and Investigating Developmental Processes Through Live Imaging
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    Chapter 6 Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement
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    Chapter 7 Visualizing Microtubule Networks During Drosophila Oogenesis Using Fixed and Live Imaging
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    Chapter 8 Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers
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    Chapter 9 Single-Molecule RNA In Situ Hybridization (smFISH) and Immunofluorescence (IF) in the Drosophila Egg Chamber.
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    Chapter 10 Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries
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    Chapter 11 Ultrastructural Analysis of Drosophila Ovaries by Electron Microscopy
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    Chapter 12 Immuno-Electron Microscopy and Electron Microscopic In Situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries
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    Chapter 13 Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry
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    Chapter 14 Detection of Cell Death and Phagocytosis in the Drosophila Ovary
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    Chapter 15 Analysis of Cell Cycle Switches in Drosophila Oogenesis
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    Chapter 16 Drosophila Oogenesis
  18. Altmetric Badge
    Chapter 17 Drosophila Oogenesis
Attention for Chapter 16: Drosophila Oogenesis
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Chapter title
Drosophila Oogenesis
Chapter number 16
Book title
Drosophila Oogenesis
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2851-4_16
Pubmed ID
Book ISBNs
978-1-4939-2850-7, 978-1-4939-2851-4

Rozhkov, Nikolay V, Nikolay V. Rozhkov


In the past decade, deep-sequencing approaches have greatly improved our knowledge of the genome's potential and have become a crucial milestone for new discoveries in genomics. Transcription is the first step of gene expression; therefore, the detection and measurement of transcription rates is of great interest. Here, a detailed protocol for global run-on sequencing (GRO-seq) library preparation from Drosophila ovaries is described. The method relies on rapid isolation of nuclei with halted transcription, then restarting transcription in physiological conditions in the presence of a labeled nucleotide. The newly transcribed nascent RNA is then isolated and cloned using a small RNA cloning protocol. Although it is time-consuming, the global run-on method allows the user to profile the position, orientation and amount of transcriptionally engaged RNA polymerases across the genome, therefore providing a snapshot of genome-wide transcription.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Finland 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 33%
Student > Bachelor 2 17%
Student > Master 2 17%
Researcher 1 8%
Unknown 3 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 50%
Biochemistry, Genetics and Molecular Biology 3 25%
Unknown 3 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 May 2016.
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