Chapter title |
Altering Entry Site Preference of Lentiviral Vectors into Neuronal Cells by Pseudotyping with Envelope Glycoproteins.
|
---|---|
Chapter number | 12 |
Book title |
Gene Therapy for Neurological Disorders
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3271-9_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3270-2, 978-1-4939-3271-9
|
Authors |
Kobayashi, Kenta, Kato, Shigeki, Inoue, Ken-Ichi, Takada, Masahiko, Kobayashi, Kazuto, Kenta Kobayashi, Shigeki Kato, Ken-ichi Inoue, Masahiko Takada, Kazuto Kobayashi, Inoue, Ken-ichi |
Abstract |
A lentiviral vector system provides a powerful strategy for gene therapy trials against a variety of neurological and neurodegenerative disorders. Pseudotyping of lentiviral vectors with different envelope glycoproteins not only confers the neurotropism to the vectors, but also alters the preference of sites of vector entry into neuronal cells. One major group of lentiviral vectors is a pseudotype with vesicular stomatitis virus glycoprotein (VSV-G) that enters preferentially cell body areas (somata/dendrites) of neurons and transduces them. Another group contains lentiviral vectors pseudotyped with fusion envelope glycoproteins composed of different sets of rabies virus glycoprotein and VSV-G segments that enter predominantly axon terminals of neurons and are transported through axons retrogradely to their cell bodies, resulting in enhanced retrograde gene transfer. This retrograde gene transfer takes a considerable advantage of delivering the transgene into neuronal cell bodies situated in regions distant from the injection site of the vectors. The rational use of these two vector groups characterized by different entry mechanisms will further extend the strategy for gene therapy of neurological and neurodegenerative disorders. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 32% |
Student > Master | 3 | 14% |
Student > Ph. D. Student | 3 | 14% |
Professor > Associate Professor | 2 | 9% |
Student > Bachelor | 2 | 9% |
Other | 3 | 14% |
Unknown | 2 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 5 | 23% |
Agricultural and Biological Sciences | 3 | 14% |
Biochemistry, Genetics and Molecular Biology | 3 | 14% |
Veterinary Science and Veterinary Medicine | 2 | 9% |
Immunology and Microbiology | 2 | 9% |
Other | 5 | 23% |
Unknown | 2 | 9% |