Chapter title |
AAV-Mediated Gene Transfer to Dorsal Root Ganglion
|
---|---|
Chapter number | 18 |
Book title |
Gene Therapy for Neurological Disorders
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3271-9_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3270-2, 978-1-4939-3271-9
|
Authors |
Hongwei Yu, Gregory Fischer, Quinn H. Hogan, Yu, Hongwei, Fischer, Gregory, Hogan, Quinn H. |
Abstract |
Transferring genetic molecules into the peripheral sensory nervous system to manipulate nociceptive pathophysiology is a powerful approach for experimental modulation of sensory signaling and potentially for translation into therapy for chronic pain. This can be efficiently achieved by the use of recombinant adeno-associated virus (rAAV) in conjunction with nociceptor-specific regulatory transgene cassettes. Among different routes of delivery, direct injection into the dorsal root ganglia (DRGs) offers the most efficient AAV-mediated gene transfer selectively into the peripheral sensory nervous system. Here, we briefly discuss the advantages and applications of intraganglionic microinjection, and then provide a detailed approach for DRG injection, including a list of the necessary materials and description of a method for performing DRG microinjection experiments. We also discuss our experience with several adeno-associated virus (AAV) options for in vivo transgene expression in DRG neurons. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 43 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 19% |
Researcher | 6 | 14% |
Student > Doctoral Student | 4 | 9% |
Professor > Associate Professor | 4 | 9% |
Student > Bachelor | 3 | 7% |
Other | 10 | 23% |
Unknown | 8 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 15 | 35% |
Agricultural and Biological Sciences | 8 | 19% |
Biochemistry, Genetics and Molecular Biology | 5 | 12% |
Medicine and Dentistry | 3 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 2 | 5% |
Unknown | 9 | 21% |