Chapter title |
Genomic DNA Library Preparation for Resistance Gene Enrichment and Sequencing (RenSeq) in Plants.
|
---|---|
Chapter number | 22 |
Book title |
Plant-Pathogen Interactions
|
Published in |
Methods in molecular biology, March 2014
|
DOI | 10.1007/978-1-62703-986-4_22 |
Pubmed ID | |
Book ISBNs |
978-1-62703-985-7, 978-1-62703-986-4
|
Authors |
Jupe F, Chen X, Verweij W, Witek K, Jones JD, Hein I, Florian Jupe, Xinwei Chen, Walter Verweij, Kamel Witek, Jonathan D. G. Jones, Ingo Hein, Jupe, Florian, Chen, Xinwei, Verweij, Walter, Witek, Kamel, Jones, Jonathan D. G., Hein, Ingo |
Abstract |
Enrichment of genomic DNA for genes of interest prior to high-throughput sequencing offers an efficient and cost-effective approach to reduce genome complexity. Target enrichment typically yields higher read-depth for selected genes and is therefore suitable for determination of sequence polymorphisms and enables multiplexing of samples. Target enrichment also provides a means to annotate specific gene families within the sequenced organisms without the requirements for gene models. Here we describe enrichment procedures for NB-LRR-type plant resistance genes that can, for example, be used to establish the NB-LRR gene complements of individual plants and to map resistances more rapidly using a bulked segregant analysis. |
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