Attention for Chapter 21:
Measuring Influenza Virus Infection Using Bioluminescent Reporter Viruses for In Vivo Imaging and In Vitro Replication Assays
Altmetric Badge
About this Attention Score
Average Attention Score compared to outputs of the same age and source
Erik A. Karlsson, Victoria A. Meliopoulos, Vy Tran, Chandra Savage, Brandi Livingston, Stacey Schultz-Cherry, Andrew Mehle, Karlsson, Erik A., Meliopoulos, Victoria A., Tran, Vy, Savage, Chandra, Livingston, Brandi, Schultz-Cherry, Stacey, Mehle, Andrew
Abstract
To streamline standard virological assays, we developed bioluminescent replication-competent influenza reporter viruses that mimic their parental counterparts. These reporter viruses provide a rapid and quantitative readout of viral infection and replication. Moreover, they permit real-time in vivo measures of viral load, tissue distribution, and transmission in the same cohort of animals over the entire course of infection-measurements that were not previously possible. Here we provide detailed protocols using bioluminescent reporter viruses for in vivo imaging in mice and ferrets. We also describe cell culture-based techniques using reporter viruses for quantification of viral titers and performing microneutralization assays. The ease, speed, and adaptability of these approaches have the potential to accelerate multiple areas of influenza virus research.
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 March 2022.
All research outputs
#15,778,526
of 23,435,471 outputs
Outputs from Methods in molecular biology
#5,498
of 13,324 outputs
Outputs of similar age
#213,240
of 335,701 outputs
Outputs of similar age from Methods in molecular biology
#101
of 248 outputs
Altmetric has tracked 23,435,471 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,324 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 335,701 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 248 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
