Chapter title |
Metal-Tagging Transmission Electron Microscopy and Immunogold Labeling on Tokuyasu Cryosections to Image Influenza A Virus Ribonucleoprotein Transport and Packaging
|
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Chapter number | 14 |
Book title |
Influenza Virus
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8678-1_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8677-4, 978-1-4939-8678-1
|
Authors |
Martin Sachse, Isabel Fernández de Castro, Guillaume Fournier, Nadia Naffakh, Cristina Risco |
Abstract |
Transmission electron microscopy (TEM) has been instrumental for studying viral infections. In particular, methods for labeling macromolecules at the ultrastructural level, by integrating biochemistry, molecular biology, and morphology, have allowed to study the functions of viral macromolecular complexes within the cellular context. Here, we describe a strategy for imaging influenza virus ribonucleoproteins in infected cells with two complementary labeling methods, metal-tagging transmission electron microscopy or METTEM, a highly sensitive technique based on the use of a metal-binding protein as a clonable tag, and immunogold labeling on thawed cryosections, a very specific labeling method that allows to study the distribution of different proteins simultaneously. The combination of both labeling methods offers new possibilities for TEM analysis of viral components in cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 42% |
Researcher | 4 | 33% |
Other | 1 | 8% |
Student > Master | 1 | 8% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 33% |
Veterinary Science and Veterinary Medicine | 2 | 17% |
Agricultural and Biological Sciences | 2 | 17% |
Medicine and Dentistry | 2 | 17% |
Immunology and Microbiology | 1 | 8% |
Other | 0 | 0% |
Unknown | 1 | 8% |