| Chapter title |
Purification and Proteomics of Influenza Virions
|
|---|---|
| Chapter number | 5 |
| Book title |
Influenza Virus
|
| Published in |
Methods in molecular biology, August 2018
|
| DOI | 10.1007/978-1-4939-8678-1_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8677-4, 978-1-4939-8678-1
|
| Authors |
Edward C. Hutchinson, Monika Stegmann, Hutchinson, Edward C., Stegmann, Monika |
| Abstract |
This chapter describes a basic workflow for analyzing the protein composition of influenza virions. In order to obtain suitable material, the chapter describes how to concentrate influenza virions from the growth media of infected cells and to purify them by ultracentrifugation through a density gradient. This approach is also suitable for purifying influenza virions from the allantoic fluid of embryonated chicken eggs. As a small quantity of microvesicles are co-purified with virions, optional steps are included to increase the stringency of purification by enriching material with viral receptor binding and cleaving activity. Material purified in this way can be used for a variety of downstream applications, including proteomics. As a detailed example of this, the chapter also describes a standard workflow for analyzing the protein composition of concentrated virions by liquid chromatography and tandem mass spectrometry. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 29% |
| Researcher | 7 | 21% |
| Student > Master | 4 | 12% |
| Student > Bachelor | 3 | 9% |
| Other | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 8 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 29% |
| Immunology and Microbiology | 5 | 15% |
| Agricultural and Biological Sciences | 4 | 12% |
| Veterinary Science and Veterinary Medicine | 2 | 6% |
| Business, Management and Accounting | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 10 | 29% |