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Mendeley readers
Chapter title |
Live Imaging of Influenza Viral Ribonucleoproteins Using Light-Sheet Microscopy
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Chapter number | 15 |
Book title |
Influenza Virus
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Published in |
Methods in molecular biology, August 2018
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DOI | 10.1007/978-1-4939-8678-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8677-4, 978-1-4939-8678-1
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Authors |
Amar R. Bhagwat, Valerie Le Sage, Seema S. Lakdawala |
Abstract |
Influenza viruses exhibit a complex life cycle that is still poorly understood. It involves independent replication of each of the eight segments that make up its genome and subsequent coordinated assembly as they egress from the host cell. Fast, time-resolved volumetric live cell imaging offers a powerful tool for understanding the various host mechanisms hijacked by the virus. Here, we describe the methods necessary for generating influenza viruses that carry a fluorescently tagged polymerase complex, infection of biologically relevant cells with these viruses, and finally protocols for live cell imaging and analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 55% |
Researcher | 3 | 27% |
Student > Bachelor | 1 | 9% |
Student > Doctoral Student | 1 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 45% |
Agricultural and Biological Sciences | 2 | 18% |
Immunology and Microbiology | 2 | 18% |
Veterinary Science and Veterinary Medicine | 1 | 9% |
Engineering | 1 | 9% |
Other | 0 | 0% |