Chapter title |
Quantitative Determination of DNA Bridging Efficiency of Chromatin Proteins
|
---|---|
Chapter number | 12 |
Book title |
Bacterial Chromatin
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8675-0_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8674-3, 978-1-4939-8675-0
|
Authors |
Ramon A. van der Valk, Liang Qin, Geri F. Moolenaar, Remus T. Dame, Valk, Ramon A. van der, Qin, Liang, Moolenaar, Geri F., Dame, Remus T. |
Abstract |
DNA looping is important for genome organization in all domains of life. The basis of DNA loop formation is the bridging of two separate DNA double helices. Detecting DNA bridge formation generally involves the use of complex single-molecule techniques (atomic force microscopy, magnetic, or optical tweezers). Although DNA bridging can be qualitatively described, quantification of DNA bridging and bridging dynamics using these techniques is challenging. Here, we describe a novel biochemical assay capable of not only detecting DNA bridge formation, but also allowing for quantification of DNA bridging efficiency and the effects of physico-chemical conditions on DNA bridge formation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor > Associate Professor | 2 | 25% |
Student > Master | 2 | 25% |
Student > Postgraduate | 1 | 13% |
Other | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 63% |
Physics and Astronomy | 1 | 13% |
Unknown | 2 | 25% |