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Mendeley readers
Chapter title |
Genetic Approaches to Study the Interplay Between Transcription and Nucleoid-Associated Proteins in Escherichia coli
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Chapter number | 8 |
Book title |
Bacterial Chromatin
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Published in |
Methods in molecular biology, August 2018
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DOI | 10.1007/978-1-4939-8675-0_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8674-3, 978-1-4939-8675-0
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Authors |
Karin Schnetz, Schnetz, Karin |
Abstract |
Bacterial nucleoid-associated proteins are important in nucleoid-structuring, homeostasis of DNA supercoiling, and in regulation of transcription. Vice versa, transcription influences DNA supercoiling and possibly DNA-binding of nucleoid-associated proteins. Here, I describe genetic tools to study the interplay between transcription and nucleoid-associated proteins such as H-NS in Escherichia coli. These standard methods include construction of genomic promoter reporter gene fusions to study regulation of promoters, genome insertion of promoter cassettes to drive expression of a gene of interest, and construction of isogenic hns mutants and precautions when doing so. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 50% |
Researcher | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Agricultural and Biological Sciences | 1 | 25% |
Immunology and Microbiology | 1 | 25% |
Unknown | 1 | 25% |