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Bacterial Chromatin

Overview of attention for book
Cover of 'Bacterial Chromatin'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Determination of the 3D Genome Organization of Bacteria Using Hi-C
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    Chapter 2 Processing and Analysis of Hi-C Data on Bacteria
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    Chapter 3 GeF-seq: A Simple Procedure for Base Pair Resolution ChIP-seq
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    Chapter 4 Genomic SELEX Screening of Regulatory Targets of Escherichia coli Transcription Factors
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    Chapter 5 Modular Assembly of Synthetic Secondary Chromosomes
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    Chapter 6 High-Resolution Characterization of DNA/Protein Complexes in Living Bacteria
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    Chapter 7 Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor Three-Dimensional Superresolution Structured Illumination Microscopy
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    Chapter 8 Genetic Approaches to Study the Interplay Between Transcription and Nucleoid-Associated Proteins in Escherichia coli
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    Chapter 9 Atomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organization
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    Chapter 10 Dynamic Light Scattering of DNA-Ligand Complexes
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    Chapter 11 Microscale Thermophoresis Analysis of Chromatin Interactions
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    Chapter 12 Quantitative Determination of DNA Bridging Efficiency of Chromatin Proteins
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    Chapter 13 Approaches for Determining DNA Persistence Length Using Atomic Force Microscopy
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    Chapter 14 Quantitation of DNA-Binding Affinity Using Tethered Particle Motion
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    Chapter 15 Observing Bacterial Chromatin Protein-DNA Interactions by Combining DNA Flow-Stretching with Single-Molecule Imaging
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    Chapter 16 Unraveling the Biophysical Properties of Chromatin Proteins and DNA Using Acoustic Force Spectroscopy
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    Chapter 17 Unraveling DNA Organization with Single-Molecule Force Spectroscopy Using Magnetic Tweezers
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    Chapter 18 In Vitro Transcription Assay to Quantify Effects of H-NS Filaments on RNA Chain Elongation by RNA Polymerase
  20. Altmetric Badge
    Chapter 19 Deciphering 3D Organization of Chromosomes Using Hi-C Data
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    Chapter 20 Molecular Dynamics Simulation of a Feather-Boa Model of a Bacterial Chromosome
Attention for Chapter 10: Dynamic Light Scattering of DNA-Ligand Complexes
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Chapter title
Dynamic Light Scattering of DNA-Ligand Complexes
Chapter number 10
Book title
Bacterial Chromatin
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8675-0_10
Pubmed ID
30109611
Book ISBNs
978-1-4939-8674-3, 978-1-4939-8675-0
Authors

Guangcan Yang, Yanwei Wang, Yang, Guangcan, Wang, Yanwei

Abstract

Dynamic Light Scattering ( DLS ) enables the characterization of sizes and electrokinetic properties of colloids, polymers, macromolecules. DNA is a charged semiflexible polyelectrolyte, which is condensed or compacted by counterions, proteins, and other condensing agents in processes such as chromosome compaction and gene therapeutic applications. DNA condensation is closely related to charge screening, since packaging requires effective neutralization of its surface negative charges. In this chapter, we describe in detail the protocol for DLS of DNA-ligand complexes. As an example, we describe data for condensation of DNA by chitosan and the measurement of size, zeta potential, and electrophoretic mobility of the DNA-ligand complex by DLS.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 17%
Student > Bachelor 2 11%
Professor > Associate Professor 2 11%
Student > Ph. D. Student 2 11%
Librarian 1 6%
Other 2 11%
Unknown 6 33%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 4 22%
Chemistry 3 17%
Biochemistry, Genetics and Molecular Biology 2 11%
Linguistics 1 6%
Agricultural and Biological Sciences 1 6%
Other 1 6%
Unknown 6 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2018.
All research outputs
#20,530,891
of 23,100,534 outputs
Outputs from Methods in molecular biology
#9,977
of 13,208 outputs
Outputs of similar age
#288,617
of 330,630 outputs
Outputs of similar age from Methods in molecular biology
#186
of 245 outputs
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So far Altmetric has tracked 13,208 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 245 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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