| Chapter title |
High-Throughput Extraction and Detection of Drugs in Urine: Parallel Sampling with Solid-Phase Microextraction (SPME) Fibers Coupled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS) Detection
|
|---|---|
| Chapter number | 9 |
| Book title |
Analysis of Drugs of Abuse
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8579-1_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8578-4, 978-1-4939-8579-1
|
| Authors |
Frederick Li, Brian Musselman, Li, Frederick, Musselman, Brian |
| Abstract |
Determination of drugs of abuse in urine is routinely accomplished by utilizing solid-phase extraction to isolate the drugs and gas chromatography/mass spectrometry (GC/MS) for their detection. Although robotic systems are employed, throughput is limited by the extraction process and GC chromatographic separation.A method that utilizes an array of 12 solid-phase microextraction (SPME) fibers for simultaneous isolation of drugs of abuse from urine is provided as a means to increase productivity. A SPME probe holder that permits movement of up to 12 fibers through the various steps of the extraction process in parallel is utilized. Use of an automated stage for fiber presentation into the ionization region of a Direct Analysis in Real Time equipped LC/MS facilitates rapid interrogation of each SPME. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 20% |
| Unspecified | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Researcher | 1 | 10% |
| Lecturer | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 4 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 4 | 40% |
| Unspecified | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Unknown | 4 | 40% |