| Chapter title |
UPLC-Orbitrap® Screening for over 35 Drugs of Abuse and Metabolites in Biological Fluids in Under 10 min
|
|---|---|
| Chapter number | 7 |
| Book title |
Analysis of Drugs of Abuse
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8579-1_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8578-4, 978-1-4939-8579-1
|
| Authors |
Eshwar Jagerdeo, Jason E. Schaff, Jagerdeo, Eshwar, Schaff, Jason E. |
| Abstract |
We present a UPLC®-High Resolution Mass Spectrometric method to simultaneously screen for 19 benzodiazepines, 12 opiates, cocaine and three metabolites, and 3 "Z-drug" hypnotic sedatives in both blood and urine specimens. Sample processing consists of a high-speed, high-temperature enzymatic hydrolysis for urine samples followed by a rapid supported liquid extraction (SLE). The combination of ultrahigh-resolution chromatography with high resolution mass spectrometry allows all analytes to be uniquely detected with a 10 min analytical run. Limits of detection for all target analytes are 3 ng/mL or better, with only 300 μL of specimen used for analysis. The combination of low sample volume with fast processing and analysis makes this method a suitable replacement for immunoassay screening of the targeted drug classes, while providing far superior specificity and better limits of detection than can routinely be obtained by immunoassay. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 33% |
| Student > Doctoral Student | 1 | 11% |
| Lecturer | 1 | 11% |
| Student > Ph. D. Student | 1 | 11% |
| Student > Postgraduate | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 2 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 22% |
| Chemistry | 2 | 22% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Medicine and Dentistry | 1 | 11% |
| Psychology | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 2 | 22% |