Chapter title |
Methods for Expression and Purification of Biologically Active Recombinant Progranulin
|
---|---|
Chapter number | 3 |
Book title |
Progranulin
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8559-3_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8557-9, 978-1-4939-8559-3
|
Authors |
Chun Yu Zhao, Andrew Bateman, Zhao, Chun Yu, Bateman, Andrew |
Abstract |
Purifying progranulin may be useful in a variety of situations, for example, after it has been mutated or otherwise modified or when working with a species for which commercially produced progranulin is unavailable. A method to express and purify human progranulin is presented. Progranulin is transiently expressed in mammalian cells and isolated from their conditioned medium before purification by reversed-phase high-performance liquid chromatography (RP-HPLC). Human progranulin is used as an example, but the protocol can be applied to any other progranulin protein. Modifications of the expression-purification strategy for metabolic labeling of progranulin and analytical systems based on heparin-affinity chromatography are presented. |
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Geographical breakdown
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 1 | 33% |
Unknown | 2 | 67% |
Readers by discipline | Count | As % |
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Chemistry | 1 | 33% |
Unknown | 2 | 67% |