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Progranulin

Overview of attention for book
Cover of 'Progranulin'

Table of Contents

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    Book Overview
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    Chapter 1 A Brief Overview of Progranulin in Health and Disease
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    Chapter 2 Chromatographic Methods for the Purification of Granulin Peptides
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    Chapter 3 Methods for Expression and Purification of Biologically Active Recombinant Progranulin
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    Chapter 4 Large-Scale Generation of Recombinant Granulin Peptides in E. coli
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    Chapter 5 Nuclear Magnetic Resonance Spectroscopy in Analysis of Granulin Three-Dimensional Structure and Cysteine Bridging
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    Chapter 6 Data Mining: Applying the AD&FTD Mutation Database to Progranulin
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    Chapter 7 Measurement of Circulating Progranulin (PGRN/GP88/GEP) by Enzyme-Linked Immunosorbent Assay and Application in Human Diseases
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    Chapter 8 Immunohistochemical Detection of Progranulin (PGRN/GP88/GEP) in Tumor Tissues as a Cancer Prognostic Biomarker
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    Chapter 9 Analysis of Progranulin-Mediated Akt and MAPK Activation
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    Chapter 10 Mouse Monoclonal Antibodies Against Progranulin (PGRN/GEP) as Therapeutics in Preclinical Cancer Models
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    Chapter 11 Methods to Analyze the Role of Progranulin (PGRN/GEP) on Cancer Stem Cell Features
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    Chapter 12 Methods to Study the Role of Progranulin in the Tumor Microenvironment
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    Chapter 13 Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo Using Caenorhabditis elegans
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    Chapter 14 The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response
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    Chapter 15 Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function
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    Chapter 16 Methods to Investigate the Molecular Basis of Progranulin Actions on Brain and Behavior In Vivo Using Knockout Mice
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    Chapter 17 Methods to Investigate the Protection Against Neurodegenerative Disorders Provided by Progranulin Gene Transfer in the Brain
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    Chapter 18 The Interaction Between Progranulin with Sortilin and the Lysosome
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    Chapter 19 Methods to Study the Role of Progranulin in Preimplantation Mouse Embryo Development
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    Chapter 20 Establishment of a Modified Collagen-Induced Arthritis Mouse Model to Investigate the Anti-inflammatory Activity of Progranulin in Inflammatory Arthritis
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    Chapter 21 Methods for Studying the Function of Progranulin in Atherosclerosis Using Both Knockout Mice Models and In Vitro Studies
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    Chapter 22 Methods to Investigate the Roles of Progranulin in Angiogenesis Using In Vitro Strategies and Transgenic Mouse Models
Attention for Chapter 13: Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo Using Caenorhabditis elegans
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Chapter title
Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo Using Caenorhabditis elegans
Chapter number 13
Book title
Progranulin
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8559-3_13
Pubmed ID
Book ISBNs
978-1-4939-8557-9, 978-1-4939-8559-3
Authors

James Julian Doyle, J. Alex Parker, Doyle, James Julian, Parker, J. Alex

Abstract

The C. elegans nematode is a powerful genetic tool for the study of aging, developmental biology, and neurodegenerative diseases. They are a small and simple model, but its well-known genome and presence of many human orthologs has made it an ideal model to study the effects of PGRN in vivo. Here, we will describe the protocols used by our laboratory to study how PGRN affects C. elegans neuronal morphology and locomotion behavior through the use of a loss-of-function model of the nematode otholog of the GRN gene, pgrn-1. Although these protocols have been used by us to specifically study the pgrn-1 gene, the experiments are applicable to any of the animal's genes.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 2 40%
Student > Ph. D. Student 1 20%
Researcher 1 20%
Unknown 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 40%
Agricultural and Biological Sciences 2 40%
Unknown 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 June 2018.
All research outputs
#18,640,437
of 23,092,602 outputs
Outputs from Methods in molecular biology
#7,988
of 13,207 outputs
Outputs of similar age
#330,857
of 442,643 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,499 outputs
Altmetric has tracked 23,092,602 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,207 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,643 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.