Report for: Progranulin

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Chapter title	Methods for Expression and Purification of Biologically Active Recombinant Progranulin
Chapter number	3
Book title	Progranulin
Published in	Methods in molecular biology, January 2018
DOI	10.1007/978-1-4939-8559-3_3
Pubmed ID	29956267
Book ISBNs	978-1-4939-8557-9, 978-1-4939-8559-3
Authors	Chun Yu Zhao, Andrew Bateman, Zhao, Chun Yu, Bateman, Andrew
Abstract	Purifying progranulin may be useful in a variety of situations, for example, after it has been mutated or otherwise modified or when working with a species for which commercially produced progranulin is unavailable. A method to express and purify human progranulin is presented. Progranulin is transiently expressed in mammalian cells and isolated from their conditioned medium before purification by reversed-phase high-performance liquid chromatography (RP-HPLC). Human progranulin is used as an example, but the protocol can be applied to any other progranulin protein. Modifications of the expression-purification strategy for metabolic labeling of progranulin and analytical systems based on heparin-affinity chromatography are presented.

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Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	3	100%

Demographic breakdown

Readers by professional status	Count	As %
Student > Master	1	33%
Unknown	2	67%

Readers by discipline	Count	As %
Chemistry	1	33%
Unknown	2	67%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 May 2023.

All research outputs

#7,836,997

of 23,770,218 outputs

Outputs from Methods in molecular biology

#2,441

of 13,418 outputs

Outputs of similar age

#155,669

of 446,105 outputs

Outputs of similar age from Methods in molecular biology

#237

of 1,479 outputs

Altmetric has tracked 23,770,218 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.

So far Altmetric has tracked 13,418 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 75% of its peers.

Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 446,105 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.

We're also able to compare this research output to 1,479 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.

Progranulin

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Attention Score in Context