Chapter title |
The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response
|
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Chapter number | 14 |
Book title |
Progranulin
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8559-3_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8557-9, 978-1-4939-8559-3
|
Authors |
Tsung-Yuan Hsu, Victoria J. Butler, Aimee W. Kao, Hsu, Tsung-Yuan, Butler, Victoria J., Kao, Aimee W. |
Abstract |
The nematode Caenorhabditis elegans (C. elegans) has proven to be a powerful model organism for the study of many biological processes, with major implications for human health and disease. As progranulin is a pleiotropic, secreted protein with both cell autonomous and non-autonomous roles, a multicellular organism such as C. elegans is ideal for the investigation of its normal function and pathological effects. The C. elegans genome contains a progranulin-like gene known as pgrn-1. The nematode pgrn-1 encodes a protein with three cysteine-rich granulin domains, compared to the seven and a half granulins in the human protein. We have shown that C. elegans mutants lacking pgrn-1 appear grossly normal, but exhibit accelerated apoptotic cell engulfment as well as a stress resistance phenotype (Kao et al., Proc Natl Acad Sci U S A 108:4441-4446, 2011; Judy et al., PLoS Genet 9:e1003714, 2013). In addition, the roles of individual granulins can also be dissected in C. elegans (Salazar et al., J Neurosci 35:9315-9328, 2015). Here, we describe methods for studying apoptosis and stress response in C. elegans. |
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