Chapter title |
Mass Spectrometry of Gangliosides
|
---|---|
Chapter number | 9 |
Book title |
Gangliosides
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8552-4_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8551-7, 978-1-4939-8552-4
|
Authors |
Akemi Suzuki, Minoru Suzuki, Emi Ito, Takahiro Nitta, Jin-ichi Inokuchi, Suzuki, Akemi, Suzuki, Minoru, Ito, Emi, Nitta, Takahiro, Inokuchi, Jin-ichi |
Abstract |
This chapter describes protocols for mass spectrometry (MS) applied to the characterization of ganglioside structures and the determination of ganglioside contents. Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) are often used to ionize biological materials and this chapter covers three protocols for atmospheric pressure MALDI MS (AP-MALDI MS), liquid chromatography-ESI MS (LC-ESI MS), and LC-ESI MS with multiple reaction monitoring (MRM). Purified gangliosides were used in AP-MALDI MS analyses while crude preparations of gangliosides were subjected to LC-ESI MS and LC-ESI MS with MRM. The LC protocol includes conditions for both reversed-phase and normal-phase column chromatography. |
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