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Chapter title |
Determination of Glycolipid Ligands of Carbohydrate-Binding Proteins Directly on Thin Layer Chromatograms
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Chapter number | 21 |
Book title |
Gangliosides
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8552-4_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8551-7, 978-1-4939-8552-4
|
Authors |
John L. Magnani |
Abstract |
Determination of a glycolipid ligand can quickly reveal the carbohydrate epitope recognized by carbohydrate-binding proteins (CBP) which may include antibodies, lectins, toxins, and virulence factors. Thin layer chromatography (TLC) is a simple, well-known, and heavily used method to separate glycolipids based on their migration through a thin layer of chromatography media, usually silica gel, coated on the surface of a rigid plate. Reacting carbohydrate-binding proteins directly on a TLC plate of separated glycolipids is a powerful technique to quickly determine functional carbohydrate epitopes present in an entire glycolipid extract of a target cell type or tissue. |