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Mendeley readers
Chapter title |
Methods for the Preparation of Anti-ganglioside Monoclonal Antibodies
|
---|---|
Chapter number | 13 |
Book title |
Gangliosides
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8552-4_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8551-7, 978-1-4939-8552-4
|
Authors |
Noriyuki Yuasa, Ikuo Kawashima |
Abstract |
The antibody preparation method to the glycolipid is basically same to the method to the protein. However, because the immunogenicity of the carbohydrate moieties of glycolipid is generally low in the mouse, the development of the anti-glycolipid antibody using purified glycolipid as an immunogen was not yet established. Here, we describe a method using a purified ganglioside adsorbed onto Salmonella minnesota for the efficient production of an anti-ganglioside mouse monoclonal antibody that recognizes the carbohydrate moieties of the ganglioside. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 67% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Medicine and Dentistry | 1 | 33% |
Unknown | 1 | 33% |