Chapter title |
Synthesis of Fluorescent Gangliosides
|
---|---|
Chapter number | 16 |
Book title |
Gangliosides
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8552-4_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8551-7, 978-1-4939-8552-4
|
Authors |
Günter Schwarzmann |
Abstract |
Labeled gangliosides are invaluable tools to study their transport and metabolism within cells as well as to determine their distribution in membranes, and their interaction with membrane lipids and proteins. Here I describe established procedures to synthesize ganglioside derivatives with a fluorescent tag either attached to its sialooligosaccharide or ceramide portion. These procedures are chosen as to minimize the integrity of the ganglioside molecule and hence, to leave their native skeleton formally intact. The α-position of the stearic acid residue is favorable for the attachment both of hydrophilic and of lipophilic dyes. In some other cases, and starting from lyso-gangliosides, procedures are described by which a fluorescent tag bound to a short acyl chain replaces the native acyl chain of gangliosides. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 33% |
Student > Postgraduate | 1 | 33% |
Student > Doctoral Student | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 33% |
Medicine and Dentistry | 1 | 33% |
Unknown | 1 | 33% |