| Chapter title |
Isolation and Identification of Innate Lymphoid Cells (ILCs) for Immunotoxicity Testing
|
|---|---|
| Chapter number | 21 |
| Book title |
Immunotoxicity Testing
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8549-4_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8548-7, 978-1-4939-8549-4
|
| Authors |
Elia D. Tait Wojno, Celine A. Beamer |
| Abstract |
Innate lymphoid cells (ILCs) comprise a family of innate immune cells that orchestrate mucosal immune responses: initiating, sustaining, and even curbing immune responses. ILCs are relatively rare (≤1% of lymphocytes in mucosal tissues), lack classical cell-surface markers, and can be divided into three subsets (type 1-3 ILCs) based on differences in cytokine production, phenotype, and developmental pathway. Because ILCs can only be identified by combinations of cell-surface markers and cytokine production, multicolor flow cytometry is the most reliable method to purify, characterize, and assess the functionality of ILCs. Here, we describe the methods for cell preparation, flow cytometric analysis, and purification of murine ILCs from the lung. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 70 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 20% |
| Researcher | 10 | 14% |
| Student > Bachelor | 10 | 14% |
| Student > Master | 8 | 11% |
| Student > Doctoral Student | 4 | 6% |
| Other | 6 | 9% |
| Unknown | 18 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 19 | 27% |
| Immunology and Microbiology | 18 | 26% |
| Agricultural and Biological Sciences | 5 | 7% |
| Medicine and Dentistry | 3 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
| Other | 3 | 4% |
| Unknown | 20 | 29% |