Huntington’s Disease
Springer New York
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Mendeley readers
Attention Score in Context
| Chapter title |
Robust Induction of DARPP32-Expressing GABAergic Striatal Neurons from Human Pluripotent Stem Cells
|
|---|---|
| Book title |
Huntington’s Disease
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7825-0_27 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7824-3, 978-1-4939-7825-0
|
| Authors |
Marija Fjodorova, Meng Li |
| Abstract |
Efficient generation of disease relevant neuronal subtypes from human pluripotent stem cells (PSCs) is fundamental for realizing their promise in disease modeling, pharmaceutical drug screening and cell therapy. Here we describe a step-by-step protocol for directing the differentiation of human embryonic and induced PSCs (hESCs and hiPSCs, respectively) toward medium spiny neurons, the type of cells that are preferentially lost in Huntington's disease patients. This method is based on a novel concept of Activin A-dependent induction of the lateral ganglionic/striatal fate using a simple monolayer culture paradigm under chemically defined conditions. Transplantable medium spiny neuron progenitors amenable for cryopreservation are produced in less than 20 days, which differentiate and mature into a high yield of dopamine- and cAMP-regulated phosphoprotein, Mr 32 kDa (DARPP32) expressing gamma-aminobutyric acid (GABA)-ergic neurons in vitro and in the adult rat brain after transplantation. This method has been validated in multiple hESC and hiPSC lines, and is independent of the regime for PSC maintenance. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 26% |
| Student > Ph. D. Student | 5 | 22% |
| Student > Master | 2 | 9% |
| Student > Bachelor | 2 | 9% |
| Unspecified | 1 | 4% |
| Other | 2 | 9% |
| Unknown | 5 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 5 | 22% |
| Agricultural and Biological Sciences | 3 | 13% |
| Biochemistry, Genetics and Molecular Biology | 2 | 9% |
| Medicine and Dentistry | 2 | 9% |
| Unspecified | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 7 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2018.
All research outputs
#15,532,144
of 23,083,773 outputs
Outputs from Methods in molecular biology
#5,409
of 13,205 outputs
Outputs of similar age
#270,117
of 442,605 outputs
Outputs of similar age from Methods in molecular biology
#596
of 1,499 outputs
Altmetric has tracked 23,083,773 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,205 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.