Huntington’s Disease
Springer New York
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Attention Score in Context
| Chapter title |
Assessing Autophagic Activity and Aggregate Formation of Mutant Huntingtin in Mammalian Cells
|
|---|---|
| Chapter number | 2 |
| Book title |
Huntington’s Disease
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7825-0_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7824-3, 978-1-4939-7825-0
|
| Authors |
Eleanna Stamatakou, Ye Zhu, David C. Rubinsztein, Stamatakou, Eleanna, Zhu, Ye, Rubinsztein, David C. |
| Abstract |
The accumulation of mutant aggregate-prone proteins is a hallmark of the majority of neurodegenerative disorders, including Alzheimer's, Parkinson's, and Huntington's diseases. Autophagy, a cytosolic bulk degradation system, is the major clearance pathway for several aggregate-prone proteins, such as mutant huntingtin. The autophagosome-associated protein LC3-II is a specific marker of autophagic flux within cells, whereas aggregate formation of mutant huntingtin represents a good readout for studying autophagy modulation. Here we describe the method of assessing autophagic flux using LC3-II western blotting and substrate clearance by expressing the N-terminal fragment of huntingtin (htt exon 1) containing an expanded polyglutamine tract in mammalian cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 15% |
| Researcher | 2 | 15% |
| Student > Bachelor | 2 | 15% |
| Student > Master | 2 | 15% |
| Unspecified | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 3 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Neuroscience | 2 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
| Unspecified | 1 | 8% |
| Computer Science | 1 | 8% |
| Other | 3 | 23% |
| Unknown | 3 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2019.
All research outputs
#3,283,696
of 23,088,369 outputs
Outputs from Methods in molecular biology
#748
of 13,204 outputs
Outputs of similar age
#74,762
of 442,629 outputs
Outputs of similar age from Methods in molecular biology
#54
of 1,499 outputs
Altmetric has tracked 23,088,369 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,204 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,629 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.